Spontaneous

Of oxygen activity of neutrophils was determined by the reaction of reduction of nitro blue tetrazolium. NBT-test is based on the absorption of phagocytes Nitroblue Tetrazolium from the medium and its subsequent recovery within the cell to diformazana. Reaction with intact neutrophils characterizes the degree of "functional stimulation" of these cells in vivo (NST-test spontaneous) rates of NBT-test in neutrophils stimulated in vitro with zymosan, opsonized and neopsonizirovannym can be considered as a criterion of activity at the voltage of the functions of oxygen (NST- test-stimulated n / c and a / h). Index of stimulation of neutrophils in all kinds of zymosan (Kao, Kan, CA): The ratio diformazan-positive cells in stimulated reaction to diformazan-positive cells in the spontaneous reaction of NBT-test [5].

In all cases at all stages of a deformed cecum was fixed planar soldering to the ventral wall in the place of its deserozirovaniya. In some animals in SPBP involved bowel loops, omentum and other mobile abdominal organs. Severity SPBP on day 3 was 2,35 ± 0,22 points, at day 7 there was a slight but statistically significant decrease in its severity (p <0,05) to 2,09 ± 0,14 points. By day 14 the severity of adhesions brand name levitra also tended to decrease (1,87 ± 0,16 points), but it is not of a statistically significant nature. Histological examination revealed the dynamic changes of maturing fibrous connective tissue with signs of decay infiltrative processes and progressive angiogenesis.

On the third day after playing SPBP there was a decrease of spontaneous NBT-test, which indirectly indicated the presence of a peripheral vein of a small absolute number of cells, which caused a massive migration to the inflammatory focus. By day 7 was observed stihaniya inflammation, respectively, reduction in migration of neutrophils. In addition, as a result of compensatory mobilization of bone marrow, peripheral vein increased number of polymorphonuclear leukocytes, in connection with which there was an increase of oxygen activity in the spontaneous NBT-test, the normalization of which proishodlo to 14 day after the simulation SPBP.

In the stimulated NBT-test neopsonizirovannym zymosan brand viagra online on the third day after operation was observed to decrease. By 7 the day of the experiment - it did not differ from that of healthy animals, and by day 14, again declined. Despite such momentum, attention is drawn to the fact that at 7 and 14 day reserve of cellular activity (RNA) in response to zymosan neopsonizirovanny progressively decreased. This is explained by the presence in the blood of young, functionally immature polymorphonuclear leukocytes are not able to adequately respond to stimulation.

A formal study of NBT test stimulated by opsonized zymosan showed its decrease at 3, 7 and 14 days after modeling SPBP, but the analysis of reserve cell activity in opsonized zymosan (CAR), it was found that the changes were the same as when stimulated neopsonizirovannym zymosan.

Discrete cells' ability to respond to various stimuli (CA) changed natural: the third day after the simulation - has not changed, as in the bloodstream was attended by mature neutrophils, to the 7 day experiment, a marked "dip" due to the presence of immature white blood cells, and to day 14 - again stabilized.

Reliable correlation between the severity of SPBP and the change of oxygen dependent functions we have not been identified.

Thus, on the third day after the simulation SPBP in peripheral blood of a marked reduction of oxygen activity of neutrophils, with the exception of the activation coefficient, which rose or were the same as in healthy animals, probably due to the massive migration of inflammatory cells, the presence cialis without prescription of bloodstream of mature neutrophils, increased provision of cellular activity. By the seventh day is observed reserve of oxygen depletion of cellular activity, which is due to compensatory activation of the bone marrow and the appearance in the blood of young, functionally immature neutrophils. On the fourteenth day a partial normalization of oxygen activity of polymorphonuclear leukocytes, which is associated with stihaniya inflammatory processes in the abdominal cavity.

Consequently, when SPBP at different stages after the simulation observed dynamic changes in the indices of immunological reactivity, in particular oxygen dependent neutrophil function. In this regard, a promising direction in the development antiadgezivnyh prophylactic drugs can be considered the use of local and resorptive immunocorrecting therapy, as well as in-depth study of the patterns of immunopathogenesis SBB.

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